Chymotrypsin CAS NO.9004-07-3

Chymotrypsin Source: Bovine (Swine) pancreas General description Chymotrypsin is also called chymotrypsinogen, which is the endopeptidase extracted and separated from the fresh pancreas of cows (or p…

Chymotrypsin Source: Bovine (Swine) pancreas General description Chymotrypsin is also called chymotrypsinogen, which is the endopeptidase extracted and separated from the fresh pancreas of cows (or pigs). Primary structure and spatial structure of α- Chymotrypsin have been clearly explained, which is a single peptide chain composed of 245 amino acid residues, with 5 pairs of disulfide linkage in the molecule. It is white and light yellow crystal or amorphous powder, and easily soluble in the water, but insoluble in organic solvent. Relative molecular mass is 24000, and the optimum PH is 8~9. It is stable at a dry state, but will be quickly deactivated in water solution, most stable in the water solution with PH3~4. When it acts on the protein, it will hydrolyze the peptide bond formed by the carboxyl of L-tyrosine and L-phenylalanine in priority. The high purity Chymotrypsin of Beijing Geyuantianrun Bio-tech Co., Ltd.. is purified by re-crystallization, and then by Ion Exchange Chromatography and ultra-filtration. Specification Items Specification Method Appearance White or almose white lyophilized powder Solvent Transparentness Confroms USP30 Loss on drying ≤ 5.0% USP30 Residue on Ignition ≤2.5% USP30 Microbial limits Confroms USP30 Trypsin ≤5% USP30 Assay Chymotrypsin≥1000 USPunits/mg powder USP30 Storage Sealed, Dark, at temperature 2-8℃1.USP /EP standard Chymotrypsin 2.White crystallize powder 3.Assay(chymotrypsin) no less than 1500u/mg 4.Porcine or bovine Chymotrypsin CAS 9004-07-3 Source Bovine (Swine) pancreasGeneral descriptionChymotrypsin is also called chymotrypsinogen, which is the endopeptidase extracted and separated from the fresh pancreas of cows (or pigs).Primary structure and spatial structure of α- Chymotrypsin have been clearly explained, which is a single peptide chain composed of 245 amino acid residues, with 5 pairs of disulfide linkage in the molecule. I1.U1.USP /EP standard 2.Porcine or bovine pancreas3.Assay no less than 1000u/mg Chymotrypsin CAS 9004-07-3Source Bovine (Swine) pancreasGeneral descriptionChymotrypsin is also called chymotrypsinogen, which is the endopeptidase extracted and separated from the fresh pancreas of cows (or pigs).Primary structure and spatial structure of α- Chymotrypsin have been clearly explained, which is a single peptide chain composed of 245 amino acid residues, with 5 pairs of disulfide linkage in the molecule. It is white and light yellow crystal or amorphous powder, and easily soluble in the water, but insoluble in organic solvent. Relative molecular mass is 24000, and the optimum PH is 8~9. It is stable at a dry state, but will be quickly deactivated in water solution, most stable in the water solution with PH3~4. When it acts on the protein, it will hydrolyze the peptide bond formed by the carboxyl of L-tyrosine and L-phenylalanine in priority.The high purity Chymotrypsin of Beijing Geyuantianrun Bio-tech Co., Ltd.. is purified by re-crystallization, and then by Chromatography and ultra-filtration.Specification ItemsSpecificationMethodAppearanceWhite crystallized powderUSP33IdentificationNo purple color develops within 3 minutesUSP33Loss on dryingNo more than 5.0%USP33Residue on IgnitionNo more than 2.5%USP33Microbial limits  Pseudomonas aeruginosaAbsentUSP33Salmonella AbsentUSP33Staphylococcus aureusAbsentUSP33Limit trypsinNot more than 1%.USP33AssayNLT 1000 USP Chymotrypsin Units/mg (calculated on the dried basis) and 90.0%-110.0% of the labeled potency.USP33 Storage Sealed, Dark, at temperature 2-8°C   ItemsSpecificationMethodDescriptionwhite crystallize powderEP6.0 IdentificationA) A purple colour developsB)No colour develops with in 3 min of mixingEP6.0Appearance of solutionSolution S is not more opalescent thanreference suspension 2EP6.0 Absorbance1) 281nm 18.5 ~ 22.52) 250nm not more than 8.0EP6.0 PH3.0-5.0EP6.0 HistamineNot more than 1µg/5.0µkatalEP6.0 Loss on dryingNot more than 5%EP6.0 Limit of trypsinNot more than 1%EP6.0 AssayNot less than 5.0 ukatal/mg, calculated on thedried basisEP6.0 Storage Sealed, Dark, at temperature 2-8°C